黃嘌呤氧化酶XOD酶活檢測(cè)方法
膽堿氧化酶活力定義:One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline。
辣根過氧化物酶活力測(cè)定方法,活力定義:One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20℃ PH:7.2
乙酰膽堿酯酶活性測(cè)定方法,酶活力單位的定義 :在37℃,0.1mol/L pH=8.0磷酸緩沖液條件下,每分鐘分解1umol碘化硫代乙酰膽堿(ATCI)的酶量為一個(gè)乙酰膽堿酯酶活力單位。
β-葡萄糖苷酶酶活測(cè)定方法,酶活單位的定義:在測(cè)定條件下,每分鐘水解水楊苷產(chǎn)生 1μmol 還原糖(以葡萄糖計(jì))所需酶量定義為一個(gè)酶活力單位(U)。
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